Estimation of Nitrogen

Estimation of Nitrogen( Dumas And Kjeldahl’s Method)

For the accurate estimate of Nitrogen in an organic compound, there are two methods discussed below:

Dumas Method: The Nitrogen-containing organic compound, when heated with copper oxide in carbon dioxide, yields free Nitrogen in addition to C02 and H2O. If m be the mass of an organic compound, v1 be the volume of Nitrogen collected, and T1 be the room temperature, and p1 be the pressure of Nitrogen. Then:

Kjeldahl’s method: This method does not apply to compounds containing nitro& azo group. The mixture containing Nitrogen is heated with concentrated H2SO4 due to which Nitrogen gets converted to ammonium Sulphate.

Where,