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Organic Chemistry

Chromatography ,Adsorption Chromatography And Partition Chromatography

Chromatography And Adsorption Chromatography

  1. Chromatography: Chromatography is the technique of testing the purity of compounds. There are two phases in this technique: Stationary phase and mobile phase. The mixture of compounds is made stationary phase while the moving phase is called the mobile phase.
  • Adsorption Chromatography: When a mobile phase is allowed to move over a stationary phase ) adsorbent) the components of the mixture are moved by the varying distance over the stationary phase.
  • Column Chromatography: Separation of the mixture over a column of adsorbent
  • Thin layer Chromatography: A thin layer of adsorbent is spread over a glass plate of suitable size, and the mixture is applied as a spot about 2 cm above one end of the TLC plate
  • Partition Chromatography: This chromatography is based on the continuous differential partition of a component of mixture between mobile and stationary phases. Example: paper Chromatography.