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Botany

Competent Host

  • The competent host is essential for transformation with recombinant DNA. Many kinds of host cells, including E.coli, yeat , animal, and plant cell, are available for genetic engineering. DNA is a hydrophilic molecule; it cannot be made capable of taking up DNA . This is done by treating them with a specific concentration of a divalent cation, such as calcium ion.
  • In eukaryotes, transformation is replaced by the term transfection.
  • Possible methods-
  1. Electroporation
  2. Gene gun method
  3. Microinjection
  4. Chemical medicated gene transfer